| Catalog No. | HS839014 | ||||||||
| Brand | abinScience | ||||||||
| Host species | Rabbit | ||||||||
| Species reactivity | Human, Mouse, Rat | ||||||||
| Immunogen | E. coli - derived recombinant Human HIST2H2BE/H2BC21 (Pro2-Lys126). | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. | ||||||||
| Clonality | Polyclonal | ||||||||
| Isotype | IgG | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Target | H2B/q, Histone H2B-GL105, H2B-clustered histone 21, Histone H2B.q, H2BC21, Histone H2B type 2-E, HIST2H2BE, H2BFQ | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Accession | Q16778 | ||||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Product Usage Information |
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| Note | For research use only. |
Anti-HIST2H2BE/H2BC21 Polyclonal Antibody
Overview
Images
 Immunofluorescence |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting HIST2H2BE (HS839014) at a concentration of 13.2 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of HIST2H2BE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |