| Catalog No. | HV974056 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG4-kappa |
| Applications | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| Target | B7 homolog 1, B7-H1, B7H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg as determined by LAL method. |
| Accession | Q9NZQ7 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | CX-072, 2145091-51-4 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Anti-Human PD-L1 Reference Antibody (Pacmilimab, RUO)
Overview
Images
 SEC-HPLC |
SEC-HPLC detection for Research Grade Pacmilimab. |
 Bioactivity |
Detects Human CD274/PD-L1/B7-H1 in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Research Grade Pacmilimab. |
 Flow CytoMetry |
Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: HV974056) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |