Anti-Human CD276/B7-H3 Reference Antibody (Mirzotamab, RUO)

Catalog No.	HV612036
Brand	abinScience
Tested applications	WB, FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	CD276, B7-H3, Costimulatory molecule, B7H3, 4Ig-B7-H3, B7 homolog 3, CD276 antigen
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q5ZPR3
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	ABBV-155, 2229859-11-2
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

SEC-HPLC	SEC-HPLC detection for Research Grade Mirzotamab.
Bioactivity	Detects CD276/B7-H3 in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade Mirzotamab.
Flow CytoMetry	Flow-cytometry using APC anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD276 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD276 monoclonal antibody (Catalog: HV612036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-CD276 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human CD276 (Catalog No: HV612011)