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Anti-Human ICOS Reference Antibody (Vopratelimab, RUO)
Overview
Catalog No. HV702016
Brand abinScience
Host species Human
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target CD278, ICOS, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, AILIM
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession Q9Y6W8
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameJTX-2011, 2039148-04-2
Note For research use only. Not suitable for clinical or therapeutic use.
Images


Bioactivity
SEC-HPLC detection for Research Grade Vopratelimab.

SDS-PAGE
SDS-PAGE for Research Grade Vopratelimab.

Flow CytoMetry
Flow-cytometry using anti-human CD278 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD278 monoclonal antibody (Catalog: HV702016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Western blot
Western blot analysis was performed using anti-CD278/ICOS monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD278/ICOS (Catalog No: HV702011)
Lane 2: negative control