Anti-Human EpCAM/TROP1 Reference Antibody (Citatuzumab, RUO)

Catalog No.	HB104016
Brand	abinScience
Host species	Human
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target	Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, EGP, EGP314, EPCAM, Ep-CAM, Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TACSTD1, TROP1, Tumor-associated calcium signal transducer 1, hEGP314
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg as determined by LAL method.
Accession	P16422
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	VB6-845, 945228-49-9
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)

Bioactivity	Detects CD326/EPCAM in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade Citatuzumab.
Flow CytoMetry	Flow-cytometry using APC anti-human CD326 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD326 monoclonal antibody (Catalog: HB104016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human CD326 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD326 monoclonal antibody (Catalog: HB104016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD326 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD326 monoclonal antibody (Catalog: HB104016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human CD326 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD326 monoclonal antibody (Catalog: HB104016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.