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Anti-Human ErbB2/Her2 Reference Antibody (Zanidatamab, RUO)
Overview
Catalog No. HY286076
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG1-kappa/scFv-h-CH2-CH3
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target p185erbB2, HER2, NGL, Tyrosine kinase-type cell surface receptor HER2, NEU, MLN 19, Proto-oncogene Neu, MLN19, ERBB2, Proto-oncogene c-ErbB-2, CD340, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P04626
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameZW-25, ZW25, zanidatamabzovodotin, 2169946-15-8
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


Bioactivity
Detects CD340/ERBB2/HER2/NEU in indirect ELISAs.

SDS-PAGE
SDS-PAGE for Research Grade Zanidatamab.

Flow CytoMetry
Flow-cytometry using APC anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD340 monoclonal antibody (Catalog: HY286076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using PE anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD340 monoclonal antibody (Catalog: HY286076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using FITC anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD340 monoclonal antibody (Catalog: HY286076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.