| Catalog No. | HY286096 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | WB, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| Target | CD340, EC:2.7.10.1, ERBB2, HER2, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEU, NGL, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, EC:2.7.10.1, ERBB3, HER3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg as determined by LAL method. |
| Accession | P04626 & P21860 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | Bispecific, MCLA-128, PB4188, R040517, 1969309-56-5 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human ErbB2/Her2 & ErbB3/Her3 Bispecific Reference Antibody (Zenocutuzumab, RUO)
Overview
Images
 Bioactivity |
Detects CD340/ERBB2/HER2/NEU in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Research Grade Zenocutuzumab |
 Flow CytoMetry |
Flow-cytometry using anti-human ERBB3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human ERBB3 monoclonal antibody (Catalog: HY286096) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-CD340/ERBB2 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human CD340/ERBB2 (Catalog No: HY286011) |