Anti-Human GITR/TNFRSF18 Reference Antibody (Ragifilimab, RUO)

Catalog No.	HV475016
Brand	abinScience
Tested applications	WB, FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	Activation-inducible TNFR family receptor, GITR, TNFRSF18, CD357, Tumor necrosis factor receptor superfamily member 18, AITR, Glucocorticoid-induced TNFR-related protein
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q9Y5U5
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	Detects CD357/TNFRSF18/GITR in indirect ELISAs.
Flow CytoMetry	Flow-cytometry using APC anti-human CD357 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD357 monoclonal antibody (Catalog: HV475016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD357 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD357 monoclonal antibody (Catalog: HV475016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-CD357/TNFRSF18/GITR monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD357/TNFRSF18/GITR (Catalog No: HV475011) Lane 2: negative control
SDS-PAGE	SDS-PAGE for Research Grade Ragifilimab