Anti-Human EGFR Reference Antibody (Losatuxizumab, RUO)

Catalog No.	HF004136
Brand	abinScience
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P00533
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	ABT-806, PR-1316749, 1801544-27-3
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

SEC-HPLC	SEC-HPLC detection for Research Grade Losatuxizumab.
Bioactivity	Detects EGFR/ERBB1/HER1 in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade Losatuxizumab.
Flow CytoMetry	Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: HF004136, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human EGFR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFR monoclonal antibody (Catalog: HF004136) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.