| Catalog No. | HF004186 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB, G-protein coupled receptor 49, G-protein coupled receptor HG38, LGR5, GPR67, GPR49, G-protein coupled receptor 67, Leucine-rich repeat-containing G-protein coupled receptor 5 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P00533 & O75473 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | Bispecific, MCLA-158, 2213450-26-9 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human EGFR & LGR5 Bispecific Reference Antibody (Petosemtamab, RUO)
Overview
Images
 Bioactivity |
SEC-HPLC detection for Research Grade Petosemtamab. |
 SDS-PAGE |
SDS-PAGE for Research Grade Petosemtamab. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human EGFR&LGR5 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR&LGR5 monoclonal antibody (Catalog: HF004186, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |