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Anti-Human TFPI Reference Antibody (Befovacimab, RUO)
Overview
Catalog No. HY403016
Brand abinScience
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG2-lambda
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target Extrinsic pathway inhibitor, Lipoprotein-associated coagulation inhibitor, TFPI, EPI, LACI, TFPI1, Tissue factor pathway inhibitor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P10646
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


SEC-HPLC
SEC-HPLC detection for Research Grade Befovacimab.

Bioactivity
Detects TFPI in indirect ELISAs.

Flow Cytometry
Flow-cytometry using anti-human TFPI antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TFPI monoclonal antibody (Catalog HY403016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Research Grade Befovacimab