Anti-Human APP Reference Antibody (Gantenerumab, RUO)

Catalog No.	HY235036
Brand	abinScience
Host species	Human
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target	A4, ABPP, AD1, AICD-50, AICD-57, AICD-59, AID(50), AID(57), AID(59), APP, APPI, Abeta40, Abeta42, Alpha-CTF, Alpha-secretase C-terminal fragment, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, Beta-APP40, Beta-APP42, Beta-CTF, Beta-secretase C-terminal fragment, C31, C80, C83, C99, CVAP, Cerebral vascular amyloid peptide, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), PN-II, PreA4, Protease nexin-II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg as determined by LAL method.
Accession	P05067
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	R1450, 1043556-46-2
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	SEC-HPLC detection for Research Grade Gantenerumab.
SDS-PAGE	SDS-PAGE for Research Grade Gantenerumab.
Flow Cytometry	Flow-cytometry using anti-human APP antibody. SH-SY5Y cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human APP monoclonal antibody (Catalog HY235036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using APC anti-human APP antibody. SH-SY5Y cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human APP monoclonal antibody (Catalog HY235036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using PE anti-human APP antibody. SH-SY5Y cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human APP monoclonal antibody (Catalog HY235036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using FITC anti-human APP antibody. SH-SY5Y cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human APP monoclonal antibody (Catalog HY235036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.