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Anti-Human TIGIT Reference Antibody (Etigilimab, RUO)
Overview
Catalog No. HS739026
Brand abinScience
Host species Human
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target VSTM3, TIGIT, V-set and transmembrane domain-containing protein 3, VSIG9, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession Q495A1
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameOMP-313M32, 2044984-83-8
Note For research use only. Not suitable for clinical or therapeutic use.
Images


SEC-HPLC
SEC-HPLC detection for Research Grade Etigilimab.

Bioactivity
Detects TIGIT in indirect ELISAs.

SDS-PAGE
SDS-PAGE for Research Grade Etigilimab.

Flow CytoMetry
Flow-cytometry using anti-human TIGIT antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TIGIT monoclonal antibody (Catalog: HS739026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.