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Anti-Human FCRN/FCGRT Reference Antibody (Nipocalimab, RUO)
Overview
Catalog No. HX061046
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG1-lambda
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target IgG Fc fragment receptor transporter alpha chain, IgG receptor FcRn large subunit p51, FcRn, FCGRT, FCRN, Neonatal Fc receptor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P55899
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameM-281, M281, 2211985-36-1
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107)
Images


Bioactivity
SEC-HPLC detection for Research Grade Nipocalimab.

Flow CytoMetry
Flow-cytometry using FITC anti-human FCGRT antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human FCGRT monoclonal antibody (Catalog: HX061046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using FITC anti-human FCGRT antibody. U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human FCGRT monoclonal antibody (Catalog: HX061046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Research Grade Nipocalimab