下载
Anti-Human EphA3 Reference Antibody (Ifabotuzumab, RUO)
Overview
Catalog No. HB681016
Brand abinScience
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG1-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target HEK, Eph-like tyrosine kinase 1, EK4, EPHA3, Tyrosine-protein kinase receptor ETK1, Tyrosine-protein kinase TYRO4, Ephrin type-A receptor 3, hEK4, ETK, TYRO4, ETK1, Human embryo kinase, EPH-like kinase 4
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P29320
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameFibatuzumab, KB-004, 1234137-51-9
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


SEC-HPLC
SEC-HPLC detection for Research Grade Ifabotuzumab.

Bioactivity
Detects EPHA3 in indirect ELISAs.

SDS-PAGE
SDS-PAGE for Research Grade Ifabotuzumab.

Flow Cytometry
Flow-cytometry using anti-human EPHA3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EPHA3 monoclonal antibody (Catalog HB681016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.