| Catalog No. | HT073026 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | human IgG1 - kappa - lambda with half-IG VL-CH1/VH-CK crossover |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A, ANG-2, ANGPT2, Angiopoietin-2 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P15692 & O15123 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | Bispecific, RG-7221, RG7221, RO5520985, 1448221-05-3 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human VEGF & Angiopoietin-2 Bispecific Reference Antibody (Vanucizumab, RUO)
Overview
Images
 Bioactivity |
Detects Human VEGFA in indirect ELISAs. |
 SEC-HPLC |
SEC-HPLC detection for Research Grade Vanucizumab. |
 Bioactivity |
Detects Human ANGPT2 in indirect ELISAs. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human VEGFA&ANGPT2 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA&ANGPT2 monoclonal antibody (Catalog: HT073026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |