| Catalog No. | HC440026 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | WB, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | Surface-expressed protease, Gelatine degradation protease FAP, Integral membrane serine protease, Dipeptidyl peptidase FAP, Seprase, FAP, Post-proline cleaving enzyme, APCE, SIMP, FAPalpha, 170 kDa melanoma membrane-bound gelatinase, Fibroblast activation protein alpha, Serine integral membrane protease, Prolyl endopeptidase FAP |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q12884 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Anti-Human FAP & IL-2R Reference Antibody (Simlukafusp Alfa, RUO)
Overview
Images
 SEC-HPLC |
SEC-HPLC detection for Research Grade Simlukafusp alfa. |
 Bioactivity |
Detects FAP/FAPalpha in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Research Grade Simlukafusp alfa. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human FAP&IL2V antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human FAP&IL2V monoclonal antibody (Catalog: HC440026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using anti-human FAP antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human FAP monoclonal antibody (Catalog: HC440026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-FAP monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human FAP (Catalog No: HC440011), 50ng Lane 2: recombinant human FAP (Catalog No: HC440011), 30ng Lane 3: recombinant human FAP (Catalog No: HC440011), 20ng |