| Catalog No. | HW310016 |
| Brand | abinScience |
| Tested applications | WB, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P51654 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | GC33, RG7686, RO5137382, 1365267-33-9 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human Glypican 3 Reference Antibody (Codrituzumab, RUO)
Overview
Images
 SEC-HPLC |
SEC-HPLC detection for Research Grade Codrituzumab. |
 Bioactivity |
Detects GPC3 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human GPC3 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 monoclonal antibody (Catalog HW310016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Research Grade Codrituzumab. |
 Western blot |
Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human GPC3 (Catalog No: HW310011), 50ng Lane 2: recombinant human GPC3 (Catalog No: HW310011), 30ng |