Research Grade Abatacept

Catalog No.	HW630296
Brand	abinScience
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG1-Fc
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	CD80, BB1, T-lymphocyte activation antigen CD80, CD28LG, B7, CD28LG1, LAB7, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1, BU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P16410, CAS: 332348-12-6
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	SEC-HPLC detection for Research Grade Abatacept.
Flow CytoMetry	Flow-cytometry using APC anti-human CD80 &/or CD86 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD80 &/or CD86 monoclonal antibody (Catalog: HW630296, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD80 &/or CD86 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD80 &/or CD86 monoclonal antibody (Catalog: HW630296, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Research Grade Abatacept
Bioactivity	Detected by Anti-Abatacept antibody SAA2444 (Catalog No: AW630013) in indirect ELISA