Anti-Human IL-4 Reference Antibody (Vak-694, RUO)

Catalog No.

HY457026

Brand

abinScience

Tested applications

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Purity

>95% purity as determined by SDS-PAGE.

Isotype

IgG1

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Target

Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4

References

1. Koneczny I, et al. IgG4 Autoantibodies in Organ-Specific Autoimmunopathies: Reviewing Class Switching, Antibody-Producing Cells, and Specific Immunotherapies. Front Immunol. 2022;13:834342. Published 2022 Mar 24. [HY457026]
2. Pfaar O, et al. Anti-IgE: A treatment option in allergic rhinitis?. Allergol Select. 2021;5:119-127. Published 2021 Feb 24. [HY457026]

Purification

Protein A/G purified from cell culture supernatant.

Biological activity

VAK-694 binds to IL-4, reduces allergen-specific IL-4 producing cell numbers [1]. VAK-694 exerts immunomodulatory effects on TH2 memory cells [2].

Endotoxin level

< 10 EU/mg

Expression system

Mammalian cells

Accession

P05112

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative name

VAK-694, NVP-VAK-694

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

SDS-PAGE	SDS-PAGE for Research Grade VAK-694.
Bioactivity	Detects recombinant IL4 (Catalog No: HY457021) in indirect ELISA.
Western blot	Western blot analysis was performed using anti-IL4 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human IL4 (Catalog No: HY457021) Lane 2: Negative control