| Catalog No. | HW484026 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Isotype | IgG1 |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12 |
| References | 1. Braeuer RR, et al. Transcriptional control of melanoma metastasis: the importance of the tumor microenvironment. Semin Cancer Biol. 2011 Apr;21(2):83-8. [HW484026] |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | P43121 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | C3.19.1, Abx-Ma1 |
| Clone ID | Abx-Ma1 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human MCAM/CD146 Reference Antibody (Abx-Ma1, RUO)
Overview
Images
 SEC-HPLC |
SEC-HPLC detection for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1). |
 Bioactivity |
Detects CD146/MUC18/MCAM in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1). |
 Flow CytoMetry |
Flow-cytometry using APC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |