| Catalog No. | HW443013 | ||||
| Brand | abinScience | ||||
| Species reactivity | Human | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||
| Purity | >95% purity as determined by SDS-PAGE. | ||||
| Isotype | IgG1 | ||||
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo | ||||
| Target | CD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3 | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | < 10 EU/mg | ||||
| Expression system | Mammalian cells | ||||
| Accession | P32942 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Clone ID | ICM3 | ||||
| Product Usage Information |
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| Note | For research use only. Not suitable for clinical or therapeutic use. | ||||
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human ICAM-3/CD50 Reference Antibody (ICM3, RUO)
Overview
Images
 Bioactivity |
Detects Human CD50/ICAM3 in indirect ELISAs. |
 Bioactivity |
SEC-HPLC detection for Research Grade Anti-Human CD50/ICAM3 (ICM3). |
 SDS-PAGE |
SDS-PAGE for Research Grade Anti-Human CD50/ICAM3 (ICM3) |
 Flow CytoMetry |
Flow-cytometry using APC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using anti-human CD50 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |