Anti-Human CD25/IL-2R alpha Reference Antibody (Daclizumab, RUO)

Catalog No.	HF996016
Brand	abinScience
Host species	Human
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target	CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Accession	P01589
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	BIIB019, DACHYP, 152923-56-3
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

SEC-HPLC	SEC-HPLC detection for Research Grade Daclizumab.
Bioactivity	Detects Human CD25/IL2RA in indirect ELISAs.
Flow Cytometry	Flow-cytometry using anti-human IL2RA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IL2RA monoclonal antibody (Catalog HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Research Grade Daclizumab.
Flow CytoMetry	Flow-cytometry using APC anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog: HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.