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Anti-Human Integrin alpha 4/CD49d Reference Antibody (Natalizumab, RUO)
Overview
Catalog No. HY596016
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG4-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target ITGA4, CD49d, CD49 antigen-like family member D, CD49D, VLA-4 subunit alpha, Integrin alpha-IV, Integrin alpha-4
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession P13612
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameAN100226, BG00002, 189261-10-7
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG4 (S228P) Recombinant Isotype Control Antibody (SAA0184) (HP298107)
Images


Bioactivity		 SEC-HPLC detection for Research Grade Natalizumab.

SDS-PAGE		 SDS-PAGE for Research Grade Natalizumab

Flow CytoMetry		 Flow-cytometry using APC anti-human CD49d antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD49d monoclonal antibody (Catalog: HY596016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using FITC anti-human CD49d antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD49d monoclonal antibody (Catalog: HY596016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using PE anti-human CD49d antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD49d monoclonal antibody (Catalog: HY596016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.