Anti-Human TNF-alpha Reference Antibody (Certolizumab, RUO)

Catalog No.	HF879016
Brand	abinScience
Host species	Human
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	Fab'-IgG1-kappa
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target	C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form
Purification	Protein L purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Accession	P01375
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	CDP870, PHA-738144, Certolizumabpegol, 428863-50-7
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	Detects Human TNFa/TNF-alpha in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Anti-Human TNF-alpha Reference Antibody (Certolizumab, RUO)
Flow Cytometry	Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using APC anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using PE anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using FITC anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.