Anti-Human IL-17A Reference Antibody (Ixekizumab, RUO)

Catalog No.	HS856016
Brand	abinScience
Tested applications	WB, FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG4-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17A, IL17A, CTLA8, IL-17, Interleukin-17A
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q16552
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	LY2439821, 1143503-69-8
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

SEC-HPLC	SEC-HPLC detection for Research Grade Ixekizumab.
Bioactivity	Detects Human IL17A in indirect ELISAs.
Flow Cytometry	Flow-cytometry using anti-human IL17A antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL17A monoclonal antibody (Catalog HS856016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Research Grade Ixekizumab.
Western blot	Western blot analysis was performed using anti-IL17A monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human IL17A (Catalog No: HS856011), 50ng Lane 2: recombinant human IL17A (Catalog No: HS856011), 30ng Lane 3: recombinant human IL17A (Catalog No: HS856011), 20ng Lane 4: recombinant human IL17A (Catalog No: HS856011), 10ng