Anti-Human PDGF R alpha Reference Antibody (Olaratumab, RUO)

Catalog No.	HB927016
Brand	abinScience
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	PDGFR2, Platelet-derived growth factor receptor alpha, RHEPDGFRA, Alpha platelet-derived growth factor receptor, PDGFR-2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGF-R-alpha, Alpha-type platelet-derived growth factor receptor, CD140a antigen, CD140 antigen-like family member A, PDGFRA, PDGFR-alpha, CD140a
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	P16234
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	3G3, IMC-3G3, LY3012207, 1024603-93-7
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

SEC-HPLC	SEC-HPLC detection for Research Grade Olaratumab.
Bioactivity	Detects CD140a/PDGFRA in indirect ELISAs.
Flow Cytometry	Flow-cytometry using anti-human PDGFRA antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PDGFRA monoclonal antibody (Catalog HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for Research Grade Olaratumab
Flow CytoMetry	Flow-cytometry using FITC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using APC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.