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Anti-Human IL-17RA/IL-17R Reference Antibody (Brodalumab, RUO)
Overview
Catalog No. HV263016
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% purity as determined by SDS-PAGE.
Isotype IgG2-kappa
Applications ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target IL17R, CDw217, IL-17RA, Interleukin-17 receptor A, IL-17 receptor A, IL17RA, CD217
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level < 10 EU/mg
Expression system Mammalian cells
Accession Q96F46
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative nameAMG827, KHK4827, 1174395-19-7
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG2 Recombinant Isotype Control Antibody (HyHEL-10) (HV988107)
Images


Bioactivity
SEC-HPLC detection for Research Grade Brodalumab.

SDS-PAGE
SDS-PAGE for Research Grade Brodalumab.

Flow cytometry
Flow-cytometry using anti-human CD217 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD217 monoclonal antibody (Catalog HV263016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.