| Catalog No. | HV985016 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | WB, FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% purity as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Target | LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg |
| Expression system | Mammalian cells |
| Accession | Q96NY8 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | AGS-22CE, AGS-22M, AGS-22M6E, unconjugated:AGS-22C3orAGSM6, 1448664-46-7 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Anti-Human Nectin-4 Reference Antibody (Enfortumab, RUO)
Overview
Images
 SEC-HPLC |
SEC-HPLC detection for Research Grade Enfortumab. |
 Bioactivity |
Detects NECTIN4/PVRL4 in indirect ELISAs. |
 SDS-PAGE |
SDS-PAGE for Research Grade Enfortumab. |
 Flow CytoMetry |
Flow-cytometry using anti-human Nectin 4 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human Nectin 4 monoclonal antibody (Catalog: HV985016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-NECTIN4 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human NECTIN4 (Catalog No: HV985011) Lane 2: negative control |