| Catalog No. | JN842012 |
| Brand | abinScience |
| Form | Liquid |
| Storage buffer | 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol |
| Purity | >95% as determined by SDS-PAGE. |
| Applications | Removal of high mannose N-glycans from glycoproteins |
| References | 1. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31(1):2-7. [JN842012] 2. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87(20):10199-204. [JN842012] |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Protein length | PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli. |
| Nature | Recombinant |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | CAS: 83534-39-8 |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note | For research use only. |
PNGase F
Overview
Images
 SDS-PAGE |
SDS-PAGE for PNGase F |
 Bioactivity |
Deglycosylation of Human IgG1 Fc by PNGase F. |
 Bioactivity |
Schematic diagram of PNGase F cleavage site. |