Anti-Human ErbB2/Her2 Reference Antibody (Margetuximab, RUO)

Catalog No.	HY286046
Brand	abinScience
Host species	Chimeric
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1-kappa
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Target	CD340, EC:2.7.10.1, ERBB2, HER2, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEU, NGL, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg as determined by LAL method.
Accession	P04626
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	MGAH-22, 1350624-75-7
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

SEC-HPLC	SEC-HPLC detection for Research Grade Margetuximab.
Bioactivity	Detects CD340/ERBB2/HER2/NEU in indirect ELISAs.
SDS-PAGE	SDS-PAGE for Research Grade Margetuximab.
Flow CytoMetry	Flow-cytometry using APC anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD340 monoclonal antibody (Catalog: HY286046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD340 monoclonal antibody (Catalog: HY286046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD340 monoclonal antibody (Catalog: HY286046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.