| Catalog No. | HF004076 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG1-kappa |
| Applications | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| Target | EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EC:2.7.10.1, HGF receptor, HGF/SF receptor, Hepatocyte growth factor receptor, MET, Proto-oncogene c-Met, SF receptor, Scatter factor receptor, Tyrosine-protein kinase Met |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg as determined by LAL method. |
| Accession | P00533 & P08581 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | Bispecific, JNJ-61186372, JNJ-6372, CNTO-4424, JNJ61186372, JNJ-611, 2171511-58-1 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Anti-Human EGFR & HGFR/c-MET Bispecific Reference Antibody (Amivantamab, RUO)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Research Grade Amivantamab. |
 SEC-HPLC |
SEC-HPLC detection for Research Grade Amivantamab. |
 Bioactivity |
Detects EGFR/ERBB1/HER1 in indirect ELISAs. |
 Flow CytoMetry |
Flow-cytometry using APC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human EGFR monoclonal antibody (Catalog: HF004076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using PE anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR monoclonal antibody (Catalog: HF004076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: HF004076, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using anti-human EGFR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFR monoclonal antibody (Catalog: HF004076) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Bioactivity |
Detects recombinant human Met (Cat No.: HY196021) in indirect ELISA. |