| Catalog No. | VK794012 |
| Brand | abinScience |
| Form | Liquid |
| Storage buffer | 50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol |
| Purity | >95% as determined by SDS-PAGE. |
| Applications | Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP). |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Protein length | 3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli. |
| Nature | Recombinant |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Species | HRV-14 |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Predicted molecular weight | 47.46 kDa |
| Note | For research use only. |
3C Protease
Overview
Description
3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
Activity definition: 1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 μg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C.
Images
 SDS-PAGE |
SDS-PAGE for 3C Protease |