| Catalog No. | HB185056 |
| Brand | abinScience |
| Host species | Human |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. |
| Purity | >95% purity as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | (scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc |
| Applications | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| Target | Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3, T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | < 10 EU/mg as determined by LAL method. |
| Expression system | Mammalian cells |
| Accession | P20138 & P07766 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative name | Bispecific, AMG-673, 2250261-27-7 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Anti-Human Siglec-3/CD33 & CD3 epsilon Bispecific Reference Antibody (Emerfetamab, RUO)
Overview
Images
 Flow CytoMetry |
Flow-cytometry using anti-human CD33 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD33 monoclonal antibody (Catalog: HB185056, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow cytometry |
Flow-cytometry using PE anti-human CD33 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD33 monoclonal antibody (Catalog HB185056, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Research Grade Emerfetamab |