Anti-DNA Recombinant Antibody (3E10#)

Catalog No.

YP539203

Brand

abinScience

Form

Liquid

Storage buffer

0.94 mM Citric acid monohydrate, 16.7mM Trisodium citrate dihydrate, 6.2% sucrose, pH 6.5, 0.01% Tween 80

Purity

>95% as determined by SDS-PAGE.

Isotype

IgG2a, kappa

Applications

DB, ELISA, IF

Target

DNA, dsDNA

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

Please contact with the lab for this information.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

3E10#

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Note

For research use only.

RRID

Anti-dsDNA Antibody (3E10#) (abinScience Cat# YP539203, RRID:AB_3725561)

SDS-PAGE	SDS-PAGE for Anti-dsDNA Antibody (3E10#).
Immunofluorescence	Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Immunofluorescence	MG63 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting dsDNA (YP539203) overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Mouse IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of dsDNA was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).