Anti-Human DLL3 & CD3 epsilon Bispecific Reference Antibody (Tarlatamab, RUO)

Catalog No.	HP628026
Brand	abinScience
Host species	Human
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% purity as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	(scFv-heavy-kappa)-(scFv-heavy-lambda)-scFc
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target	Drosophila Delta homolog 3, Delta3, Delta-like protein 3, DLL3, T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	< 10 EU/mg
Expression system	Mammalian cells
Accession	Q9NYJ7 & P07766
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative name	Bispecific, AMG-757, 2307488-83-9
Note	For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	SEC-HPLC detection for Research Grade Tarlatamab.
SDS-PAGE	SDS-PAGE for Anti-Human DLL3 & CD3 epsilon Bispecific Reference Antibody (Tarlatamab, RUO)
Flow CytoMetry	Flow-cytometry using anti-human DLL3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human DLL3 monoclonal antibody (Catalog: HP628026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using APC anti-human DLL3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human DLL3 monoclonal antibody (Catalog HP628026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using PE anti-human DLL3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human DLL3 monoclonal antibody (Catalog HP628026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry	Flow-cytometry using FITC anti-human DLL3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human DLL3 monoclonal antibody (Catalog HP628026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.