Anti-Phospho-Tau (pS396/pS404) Recombinant Antibody (SAA0295)

Catalog No.

HY086083

Brand

abinScience

Host species

Mouse

Tested applications

FCM

Species reactivity

Human

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Purity

>95% as determined by SDS-PAGE.

Clonality

Monoclonal

Isotype

IgG2a, kappa

Applications

IHC, IP, WB, FCM

Target

MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU

Purification

Protein A/G purified from cell culture supernatant.

Endotoxin level

< 10 EU/mg

Accession

P10636

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Clone ID

SAA0295

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Note

For research use only.

RRID

Anti-Phospho-Tau (pS396/pS404) Antibody (SAA0295) (abinScience Cat# HY086083, RRID:AB_3725735)

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

SDS-PAGE	SDS-PAGE for Anti-Phospho-Tau (pS396/pS404) Antibody (SAA0295).
Flow CytoMetry	Flow-cytometry using APC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human MAPT monoclonal antibody (Catalog: HY086083, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using FITC anti-human MAPT antibody. HDLM-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human MAPT monoclonal antibody (Catalog: HY086083, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.