| Catalog No. | HY099013 | ||||||
| Brand | abinScience | ||||||
| Species reactivity | Human | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Isotype | IgG2a, kappa | ||||||
| Applications | ELISA, FCM, IF, IP, WB | ||||||
| Target | CTSL1, MEP, Procathepsin L, Major excreted protein, Cathepsin L1, CTSL | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | P07711 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Clone ID | 3D8 | ||||||
| Product Usage Information |
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| Note | For research use only. | ||||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
Anti-Human CTSL Recombinant Antibody (3D8)
Overview
Images
 Bioactivity |
Detects CTSL in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human CTSL antibody.CTSL Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CTSL antibody monoclonal antibody (Catalog # HY099013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human CTSL Antibody (3D8). |
 Western blot |
Western blot analysis was performed using anti-CTSL monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CTSL (Catalog No: HY099011) Lane 2: negative control |