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Anti-Human Gal-10/CLC Recombinant Antibody (1D11)
Overview
Catalog No. HC313013
Brand abinScience
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG2a, kappa
Applications ELISA, FCM, SPR
Target Lysolecithin acylhydrolase, Galectin-10, LGALS10A, CLC, Gal-10, LGALS10, Eosinophil lysophospholipase, Charcot-Leyden crystal protein
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession Q05315
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID1D11
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
Note For research use only.
RRID Anti-Human Gal-10/CLC Antibody (1D11) (abinScience Cat# HC313013, RRID:AB_3725832)
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images


Bioactivity
Detects Human GAL10/CLC/Galectin-10 in indirect ELISAs.

Flow Cytometry
Flow-cytometry using anti-human Galectin-10 antibody.HL-60 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-10 antibody monoclonal antibody (Catalog # HC313013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human Galectin-10 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-10 antibody monoclonal antibody (Catalog # HC313013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human Galectin-10 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-10 antibody monoclonal antibody (Catalog # HC313013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Anti-Human Gal-10/CLC Antibody (1D11).