Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact with the lab for this information.
Accession
O00182
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Flow-cytometry using anti-human Galectin-9 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human Galectin-9 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human Galectin-9 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE
SDS-PAGE for Anti-Human Gal-9/LGALS9 Antibody (G9.2-17).
Flow cytometry
Flow-cytometry using anti-human Galectin-9 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 monoclonal antibody (Catalog HT173013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.