| Catalog No. | HY276013 | ||||
| Brand | abinScience | ||||
| Species reactivity | Human | ||||
| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Isotype | IgG1, kappa | ||||
| Applications | FCM, ICC, IF, WB | ||||
| Target | Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Accession | P02771 | ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Clone ID | AF5 | ||||
| Product Usage Information |
|
||||
| Note | For research use only. | ||||
| RRID | Anti-Human AFP/Alpha-fetoprotein Antibody (AF5) (abinScience Cat# HY276013, RRID:AB_3725843) |
||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |
Anti-Human alpha-Fetoprotein/AFP Recombinant Antibody (AF5)
Overview
Images
 Bioactivity |
SEC-HPLC detection for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5). |
 Flow Cytometry |
Flow-cytometry using anti-human AFP antibody.AFP Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AFP antibody monoclonal antibody (Catalog # HY276013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5). |
 Flow CytoMetry |
Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Western blot |
Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: HY276011) Lane 2: negative control |