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InVivoMAb Anti-Human IL-2 Antibody (Iv0020)
Overview
Catalog No. HX011010
Brand abinScience
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG1
Applications ELISA, FCM, WB, Neutralization
Target IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P60568
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDIv0020
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
Images


Bioactivity		 Detects IL2 in indirect ELISAs.

Flow Cytometry		 Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # HX011010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE		 SDS-PAGE for InVivoMAb Anti-Human IL2 (Iv0020).

Western blot		 Western blot analysis was performed using anti-human IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL2 (Catalog No: HX011012)
Lane 2: negative control

Flow cytometry		 Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow cytometry		 Flow-cytometry using FITC anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human IL2 monoclonal antibody (Catalog HX011010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.