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InVivoMAb Anti-Human IL-8/CXCL8 Antibody (Iv0023)
Overview
Catalog No. HY554010
Brand abinScience
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG1, kappa
Applications ELISA, FCM, WB, Neutralization
Target GCP/IL-8 protein II, MDNCF, IL8/NAP1 form III, IL-8(1-77), Neutrophil-activating factor, GCP/IL-8 protein I, GCP/IL-8 protein VI, Interleukin-8, Chemokine (C-X-C motif) ligand 8, Neutrophil-activating protein 1, Monocyte-derived neutrophil chemotactic factor, (Ser-IL-8)72, MDNCF-b, LYNAP, GCP/IL-8 protein V, IL8/NAP1 form IV, MONAP, IL8/NAP1 form V, IL8/NAP1 form VI, NAP-1, Granulocyte chemotactic protein 1, GCP/IL-8 protein III, IL8/NAP1 form II, IL8, IL-8, CXCL8, IL8/NAP1 form I, (Ala-IL-8)77, Protein 3-10C, GCP/IL-8 protein IV, Emoctakin, GCP-1, C-X-C motif chemokine 8, NAF, T-cell chemotactic factor, MDNCF-c, Monocyte-derived neutrophil-activating peptide, Lymphocyte-derived neutrophil-activating factor
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P10145
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Clone IDIv0023
Product Usage Information
ApplicationDilution
ELISA 1:2000-1:20000
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
Images


Flow Cytometry
Flow-cytometry using anti-human IL8 antibody.IL8 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL8 antibody monoclonal antibody (Catalog # HY554010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human IL8/CXCL8 (Iv0023).

Western blot
Western blot analysis was performed using anti-IL8/CXCL8 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL8/CXCL8 (Catalog No: HY554011)
Lane 2: negative control