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InVivoMAb Anti-Human IL-18 Antibody (Iv0030)
Overview
Catalog No. HC517010
Brand abinScience
Tested applications FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG1, kappa
Applications ELISA, FCM, Neutralization
Target IFN-gamma-inducing factor, IL1F4, Interleukin-1 gamma, IL-18, IL-1 gamma, Interleukin-18, Interferon gamma-inducing factor, IGIF, IL18, Iboctadekin
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession Q14116
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDIv0030
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images


Flow Cytometry
Flow-cytometry using anti-human IL18 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # HC517010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human IL18 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # HC517010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human IL18 antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # HC517010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human IL18 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # HC517010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human IL18/IL1F4 (Iv0030).