InVivoMAb Anti-Human M-CSF Antibody (Iv0052)

Catalog No.	HY515010
Brand	abinScience
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% as determined by SDS-PAGE.
Isotype	IgG1, kappa
Applications	ELISA, FCM, Neutralization
Target	CSF1, Macrophage colony-stimulating factor 1, CSF-1, Lanimostim, M-CSF, MCSF
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	P09603
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone ID	Iv0052
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	SEC-HPLC detection for InVivoMAb Anti-Human CSF1/M-CSF (ABV0114).
Flow Cytometry	Flow-cytometry using anti-human CSF1 antibody.MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF1 antibody monoclonal antibody (Catalog # HY515010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for InVivoMAb Anti-Human CSF1/M-CSF (Iv0052).
Flow CytoMetry	Flow-cytometry using FITC anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using APC anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.