| Catalog No. | HY213010 |
| Brand | abinScience |
| Tested applications | FCM |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% as determined by SDS-PAGE. |
| Isotype | IgG1, kappa |
| Applications | ELISA, FCM, Neutralization |
| Target | Granulocyte-macrophage colony-stimulating factor, Sargramostim, GMCSF, CSF, Colony-stimulating factor, CSF2, GM-CSF, Molgramostin |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P04141 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | Iv0053 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10) (HV080407) Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
InVivoMAb Anti-Human GM-CSF Antibody (Iv0053)
Overview
Images
 Bioactivity |
SEC-HPLC detection for InVivoMAb Anti-Human CSF2/GM-CSF (ABV0027). |
 Flow Cytometry |
Flow-cytometry using anti-human CSF2 antibody.CSF2 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human CSF2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF2 antibody monoclonal antibody (Catalog # HY213010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for InVivoMAb Anti-Human CSF2/GM-CSF (Iv0053). |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CSF2 antibody. MDA-MB-231 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CSF2 monoclonal antibody (Catalog: HY213010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |