InVivoMAb Anti-Human IGF-I/IGF-1 Antibody (Iv0063)

Catalog No.	HY095010
Brand	abinScience
Tested applications	FCM
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS pH 7.4
Purity	>95% as determined by SDS-PAGE.
Isotype	IgG1, lambda
Applications	ELISA, FCM, Neutralization
Target	IGF-I, IGF1, Mechano growth factor, MGF, Insulin-like growth factor I, IBP1, Somatomedin-C
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	P05019
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone ID	Iv0063
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Bioactivity	SEC-HPLC detection for InVivoMAb Anti-Human IGF1 (ABV0025).
Flow Cytometry	Flow-cytometry using anti-human IGF2 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF2 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human IGF1 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF1 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human IGF2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF2 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry	Flow-cytometry using anti-human IGF1 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGF1 antibody monoclonal antibody (Catalog # HY095010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for InVivoMAb Anti-Human IGF1 (Iv0063).