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InVivoMAb Anti-Human beta-NGF Antibody (Iv0066)
Overview
Catalog No. HF909010
Brand abinScience
Tested applications WB, FCM
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS pH 7.4
Purity >95% as determined by SDS-PAGE.
Isotype IgG2, kappa
Applications ELISA, WB, FCM, Neutralization
Target Beta-nerve growth factor, NGFB, Beta-NGF, NGF
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P01138
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Clone IDIv0066
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG2 Recombinant Isotype Control Antibody (HyHEL-10) (HV988107)
Images


Bioactivity
SEC-HPLC detection for InVivoMAb Anti-Human NGF/Beta-NGF (ABV0233).

Flow Cytometry
Flow-cytometry using anti-human NGF antibody.NCI-H460 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NGF antibody monoclonal antibody (Catalog # HF909010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human NGF/Beta-NGF (Iv0066).

Western blot
Western blot analysis was performed using anti-NGF monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human NGF (Catalog No: HF909011)
Lane 2: negative control