| Catalog No. | HY280010 |
| Brand | abinScience |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 |
| Purity | >95% as determined by SDS-PAGE. |
| Isotype | IgG1, kappa |
| Applications | ELISA, WB, FCM, Neutralization |
| Target | HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P13500 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Clone ID | Iv0077 |
| Note | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
InVivoMAb Anti-Human CCL2/MCP-1 Antibody (Iv0077)
Overview
Images
 Bioactivity |
SEC-HPLC detection for InVivoMAb Anti-Human CCL2/MCP-1 (ABV0269). |
 Flow Cytometry |
Flow-cytometry using anti-human CCL2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human CCL2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077). |
 Western blot |
Western blot analysis was performed using anti-CCL2/MCP-1 monoclonal antibody at 1μg/ml on various samples. Lane 1: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 50ng Lane 2: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 30ng Lane 3: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 20ng Lane 4: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 10ng |