| Catalog No. | HY259013 | ||||||
| Brand | abinScience | ||||||
| Host species | Mouse | ||||||
| Tested applications | FCM | ||||||
| Species reactivity | Human, Mouse, Rat | ||||||
| Form | Liquid | ||||||
| Storage buffer | 0.01M PBS pH 7.4 | ||||||
| Purity | >95% as determined by SDS-PAGE. | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | IgG2a, kappa | ||||||
| Applications | ELISA, IF, IHC, SPR, WB, FCM | ||||||
| Target | Cold-insoluble globulin, CIG, FN, Fibronectin, FN1 | ||||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||
| Accession | P02751 | ||||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. | ||||||
| Clone ID | SAA0522 | ||||||
| Product Usage Information |
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| Note | For research use only. | ||||||
| RRID | Anti-Human FN1/Fibronectin Antibody (SAA0522) (abinScience Cat# HY259013, RRID:AB_3726039) |
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| Isotype Control | Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507) |
Anti-Human Fibronectin Recombinant Antibody (SAA0522)
Overview
Images
 SDS-PAGE |
SDS-PAGE for Anti-Human FN1/Fibronectin Antibody (SAA0522) |
 Flow cytometry |
Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FN1 monoclonal antibody (Catalog HY259013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer. |